Temperature-sensitive mutant of adenovirus type 2 blocked in virion assembly: accumulation of light intermediate particles
نویسندگان
چکیده
منابع مشابه
Characterization of a temperature-sensitive fiber mutant of type 5 adenovirus and effect of the mutation on virion assembly.
A temperature-sensitive, fiber-minus mutant of type 5 adenovirus, H5ts142, was biochemically and genetically characterized. Genetic studies revealed that H5ts142 was a member of one of the three apparent fiber complementation groups which were detected owing to intracistronic complementation. Recombination analyses showed that it occupied a unique locus at the right end of the adenovirus geneti...
متن کاملCharacterization of an early temperature-sensitive and cytocidal double mutant of adenovirus type 2.
Human adenovirus type 2 mutant, H2 ts 111, presented a double phenotype: temperature-sensitive (ts) for initiation and elongation of DNA synthesis, and cytocidal (cyt) by its large-plaque formation and the nucleolytic cleavage of both viral and cellular DNAs. Both characters were recessive since they were efficiently complemented by wild-type or other DNA-negative ts mutants. H2 ts 111 DNA--ter...
متن کاملCharacterization of a temperature-sensitive, hexon transport mutant of type 5 adenovirus.
Infection of KB cells at 39.5 degrees C with H5ts147, a temperature-sensitive (ts) mutant of type 5 adenovirus, resulted in the cytoplasmic accumulation of hexon antigen; all other virion proteins measured, however, were normally transported into the nucleus. Immunofluorescence techniques were used to study the intracellular location of viral proteins. Genetic studies revealed that H5ts147 was ...
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15 صفحه اولCryo-electron microscopy structure of adenovirus type 2 temperature-sensitive mutant 1 reveals insight into the cell entry defect.
The structure of the adenovirus type 2 temperature-sensitive mutant 1 (Ad2ts1) was determined to a resolution of 10 A by cryo-electron microscopy single-particle reconstruction. Ad2ts1 was prepared at a nonpermissive temperature and contains the precursor forms of the capsid proteins IIIa, VI, and VIII; the core proteins VII, X (mu), and terminal protein (TP); and the L1-52K protein. Cell entry...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1978
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.26.2.344-356.1978